Thomas PoulosChancellor's Professor, Molecular Biology & Biochemistry |
|
|
|
Research Interests |
Protein crystallography, protein enginering, heme enzyme structure and function. | |
| URL | Poulos Lab Web Site | |
|
Academic Distinctions |
Presidential Meritorious Service Award, Univ. Maryland 1991; Chancellor's Professor, UCI 2000 Brodie Award for Drug Metabolism Amer. Soc. of Pharmacology and Experimental Therapeutics Appointments: Research Scientist UCSD Dept. Chemistry from 1973-1983; Principal Res. Scientist at Genex Corp. from 1983-1984; Director of Protein Engineering at Genex Corp. from 1984-1987; Director for the Univ. of Maryland's Center for Advanced Res. in Biotehcnology and Professor of Chemistry, Univ. of Maryland, College Park from 1987-1991; Professor of Biochemistry & Biophysics at UCI since 1992. |
|
| Appointments |
Research Scientist UCSD Dept. Chemistry from 1973-1983; Principal Res. Scientist at Genex Corp. from 1983-1984; Director of Protein Engineering at Genex Corp. from 1984-1987; Director for the Univ. of Maryland's Center for Advanced Res. in Biotehcnology and Professor of Chemistry, Univ. of Maryland, College Park from 1987-1991; Professor of Biochemistry&Biophysics at UCI since 1992. |
|
| Publications | Li H, Shimizu H, Flinspach M, Jamal J, Yang W, Xian M, Cai T, Wen EZ, Qiang Jia Q, Wang PG, Poulos TL . (2002). The Novel Binding Mode of N-Alkyl-N'-hydroxyguanidine to meuronal nitric oxide synthase provides mechanistic Insights into NO biosynthesis Biochemistry 41, 13868-13875 | |
| Yano J, Blasco F, Li H, Schmid RD, Henne A, Poulos TL (2003). Preliminary Characterization and crystal structure of a thermostable Cytochrome P450 from Thermus thermophilus. J. Biol. Chem. 278, 608-616 | ||
| Yano J, Blasco F, Li H, Schmid RD, Henne A, Poulos TL (2003). Preliminary Characterization and crystal structure of a thermostable Cytochrome P450 from Thermus thermophilus. J. Biol. Chem. 278, 608-616 | ||
| Bhaskar B, Immoos CE, Shimizu H, Sulc F, Farmer PJ, Poulos TL (2003) A novel heme and peroxide-dependent tryptophan-tyrosine crosslink in a mutant of cytochrome c peroxidase (2003). J. Mol. Biol. 328, 157-166 | ||
| Bonagura CA, Bhaskar B, Shimizu H, Li H, Sundaramoorthy M, McRee D, Goodin DB, Poulos TL (2003). High resolution crystal structures and spectroscopy of native and Compound I cytochrome c peroxidase Biochemistry, 42, 5600-5608 | ||
| Guo M, Bhaskar B, Li H, Barrows TP, Poulos TL.(2004). Crystal structure and characterization of a cytochrome c peroxidase-cytochrome c site-specific cross-link. Proc Natl Acad Sci U S A.101, 5940-59455. | ||
| Flinspach ML, Li H, Jamal J, Yang W, Huang H, Hah JM, Gomez-Vidal JA, Litzinger EA, Silverman RB, Poulos TL. (2004) Structural basis for dipeptide amide isoform-selective inhibition of neuronal nitric oxide synthase. Nat Struct Mol Bio. 11, 54-59 | ||
| Friedman J, Lad L, Li H, Wilks A, Poulos TL. (2004) Structural basis for novel delta-regioselective heme oxygenation in the opportunistic pathogen Pseudomonas aeruginosa. Biochemistry 43,5239-5245. | ||
| Sevrioukova IF, Li H, Poulos TL. (2004) Crystal structure of putidaredoxin reductase from Pseudomonas putida, the final structural component of the cytochrome P450cam monooxygenase. J Mol Biol.336, 889-902 | ||
| Lad L, Koshkin A, Ortiz de Montellano, PR, Poulos, TL (2005). Crystal structures of the G139A, G139A-NO and G143H mutants of human heme oxygenase-1. A finely tuned hydrogen-bonding network controls oxygenase versus peroxidase activity. J. Biol. Inorg. Chem. In press | ||
| Wang J, Lad L, Poulos TL, Ortiz de Montellano PR (2005). Regiospecificity Determinants of Human Heme Oxygenase: differential nadph- and ascorbate-dependent heme cleavage by the R183E mutant. J. Biol. Chem. 280, 2797-3806. | ||
| Kuznetsov VY, Blair E, Farmer PJ, Poulos TL, Pifferitti A, Irina F. Sevrioukova IF (2005). The Putidaredoxin reductase-putidaredoxin electron transfer complex: Theoretical and experimental studies. J. Biol. Chem., 280, 16135-16142 | ||
| Sundaramoorthy M, Youngs HL, Gold MH, Poulos TL (2005). High-Resolution Crystal Structure of Manganese Peroxidase: Substrate and Inhibitor Complexes. Biochemistry 44 6463-6470. | ||
| Nagano S, Cupp-Vickery JR, Poulos TL (2005) crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and Its Mutants, A245S and A245T: investigation of the proton transfer system IN P450eryF J. Biol. Chem., 280 22102-22107. | ||
| Bhaskar B, Poulos TL (2005). The 1.13 structure of iron-free cytochrome c peroxidase. J. Biol. Inorg. Chem. 10, 425-430. | ||
| Bhaskar B, Poulos TL (2005). The 1.13 structure of iron-free cytochrome c peroxidase. J. Biol. Inorg. Chem. 10, 425-430. | ||
| Nagano S, Poulos TL (2005). Crystallographic study on the dioxygen complex of wild-type and mutant cytochrome P450cam: Implications for the dioxygen activation mechanism J. Biol. Chem. 280, 31659-31663. | ||
| Li H, Flinspach ML, Igarashi J, Jamal J, Yang W, Gmez-Vidal JA, Litzinger EA, Huang H, Erdal EP, Silverman RA, Poulos TA (2005). Exploring the binding conformations of bulkier dipeptide amide inhibitors in constitutive nitric oxide synthases. Biochemistry 44, 15222-15229 | ||
| Barrows TP, Poulos TL (2005) Role of electrostatics and salt bridges in stabilizing the compound I radical in ascorbate peroxidase. Biochemistry 44, 14062-14068. | ||
| Mbadugha BNA, Seo J., Ji H, Martasek P, Roman LJ, Shea TM, Li, Poulos, TL, Silverman RB (2006). Hydroxyl-terminated peptidomimetic inhibitors of neuronal nitric oxide synthase. Bioorg. and Med. Chem. 14, 3681-3690 | ||
| Kuchinskas M, Li H, Conrad M, , Roberts G., Poulos TL (2006). The Role of the DNA Binding Domains in CooA Activation. Biochemistry 45, 7148-7153. | ||
| Borjigin, M, Li H, Lanz ND, kerby RL, Roberts GP, Poulos TL (2007). Structure-Based Hypothesis on the Activation of the CO-sensing Transcription Factor CooA. Acta Crys D63, 282-287. | ||
| Seo J, Igarashi J, Li H, Martasek P, Roman LJ, Poulos TL, Silverman RB (2007).Structure-Based Design and Synthesis of N(omega)-Nitro-l-Arginine-Containing Peptidomimetics as Selective Inhibitors of Neuronal Nitric Oxide Synthase. Displacement of the Heme Structural Water. J Med Chem. 50, 2089-2099. | ||
| Ho WW, Li H, Nishida CR, Ortiz de Montellano PR, Poulos TL (2008) Crystal structure and properties of CYP231A2 from the thermoacidophilic archaeon Picrophilus torridus Biochemistry 47, 2071-2079 | ||
|
Professional Societies |
Protein Society American Chemical Society Ameican Crystallographic Association Biophysical Society Amer. Society of Pharmacology and Experimental Therapeutics |
|
| Graduate Programs |
Structural Biology and Molecular Biophysics Chemical Biology Medicinal Chemistry and Pharmacology |
|
| Link to this profile | http://www.faculty.uci.edu/profile.cfm?faculty_id=2699 | |
| Last updated | 04/16/2008 | |
