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Kleine Friedrich - Fluorescence wide filed and Fluorescence / Raman laser microscopy system

Epi fluorescence wide field imaging microscope, laser-based spectrometer/microscope. Raman shift cut off at 150 cm-1 to >5000 cm-1 with up to 1 cm-1 resolution. Anti-Stokes detection optional. Spectrally resolved fluorescence detection up to 1100 nm. Excitation wavelengths: 532 nm, 632.8 nm. Wide field white light all-optical imaging capability.

 

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Cary Eclipse fluorescence spectrometer

The Cary Eclipse Spectrophotometer uses a Xenon flash lamp for superior sensitivity, high signal-to-noise, and fast kinetics. It measures the emission of light from samples in four modes. Using Xenon lamp technology, it captures a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts. The Cary Eclipse is the only spectrophotometer with room light immunity. Variable controlled temperature from 0 C to 90 C. Multispeed steering.

Useful reading:

Quantum yield determination pdf

Excitation and emission spetroscopy fundamentals (by B. Herman, J.R. Lakowicz, D.B. Murphy, T.J.Fellers and M.W. Davidson for Olympus) pdf

Fluorescence spectroscopy (by Perkin Elmer) pdf

 

 

lab instructions

user guide

calibration spectrum

 

 
 

Rosencrantz and Guildenstern - Dark Field / Raman / Fluorescence microscopy system

Unique microsopy system developed by Renishaw Inc. based on thier flagmanship InVia microscope. This is high sensitivity system with integrated research grade microscopes, enabling high resolution confocal measurements both of Raman, dark field and fluorescence hyperspectral images. System employs lasers of 405 nm, 532 nm and 785 nm. Raman signal can be measured from 100 cm-1 with better than 1 cm-1 resolution capability, Raleigh scattering free. Standard filter set is optimised for very high optical efficiency. Dark Field module employ light source to cover 350 nm - 1100 nm spectrum with wide field and hyperspectral imaging as well as in-situ Raman measurements.

 

lab instructions

user guide

lasers power chart