Date: Penner Group, August 26, 2020.
This is after our physically distanced reception in Aldrich Park for Vivian (new job at KLA!) and Apurva (defended her dissertation yesterday; new job at PhageTech Inc.!). Congrats Vivian and Apurva!!

A Virus BioResistor (VBR) for Bladder Cancer Detection: Dip-and-Read Detection of DJ-1 in Urine at 10 pM in One Minute
Apurva Bhasin, Emily C. Sanders, Joshua M. Ziegler, Jeffrey S. Briggs, Nicholas P. Drago, Aisha M. Attar, Alicia M. Santos, Marie Y. True, Alana F. Ogata, Debora V. Yoon, Sudipta Majumdar, Andrew Wheat, Shae V. Patterson, Gregory A. Weiss*, Reginald M. Penner*,

Analytical Chemistry (2020) Just Accepted.

DJ-1, a 20.7 kDa protein, is overexpressed in people who have bladder cancer (BC). Its elevated concentration in urine allows it to serve as a marker for BC. But no biosensor for the detection of DJ-1 has been demonstrated. Here, we describe a virus bioresistor (VBR) capable of detecting DJ-1 in urine at a concentration of 10 pM in one minute. The VBR consists of a pair of millimeter-scale gold electrodes that measure the electrical impedance of an ultra-thin polymeric channel containing embedded virus particles. Large signal amplitudes coupled with the inherent simplicity of the VBR sensor design results in high signal-to-noise (S/N > 100) and excellent sensor-to-sensor reproducibility characterized by coefficients of variation in the range of 3-7% across the DJ-1 binding curve down to a concentration of 30 pM, near the 10 pM limit of detection (LOD), encompassing four orders of magnitude in concentration.

Electrode Degradation in Lithium-Ion Batteries
Joshua P. Pender, Gaurav Jha, Duck Hyun Youn, Joshua M. Ziegler, Ilektra Andoni, Eric J. Choi, Adam Heller, Bruce S. Dunn, Paul S. Weiss, Reginald M. Penner,* and C. Buddie Mullins*

ACS Nano 14 (2020) 1243.

Li-ion batteries have emerged as the battery of choice for electric vehicles and large-scale smart grids. Significant research efforts are devoted to identifying materials that offer higher energy density, longer cycle life, lower cost, and/or improved safety compared to those of conventional Li-ion batteries based on intercalation electrodes. By moving beyond intercalation chemistry, gravimetric capacities that are 2–5 times higher than that of conventional intercalation materials (e.g., LiCoO2 and graphite) can be achieved. The transition to higher-capacity electrode materials in commercial applications is complicated by several factors. This Review highlights the developments of electrode materials and characterization tools for rechargeable lithium-ion batteries, with a focus on the structural and electrochemical degradation mechanisms that plague these systems.

January 22, 2020
Woo-hoo! Team Biosensor revels in successful 1.0 minute VBR demo for TAE board members (raw data shown on screen). Shown are (left to right) Nick Drago, Apurva Bhasin, and Eric Choi.

Electrochemical quantification of glycated and non-glycated human serum albumin in synthetic urine
Aisha Attar, Mark Richardson, Gaetano Speciale, Sudipta Majumdar,; Rebekah Dyer, Emily Sanders, Reginald M. Penner*, Gregory Weiss*,

ACS Applied Materials and Interfaces 11 (2019) 4757.

Biomolecular damage caused by elevated serum glucose manifests in the glycation of molecules found in blood. The concentration of glycated molecules provides a direct indication of this damage, and the degree to which normal blood glucose has been elevated, over a specified time period. Aisha and her coworkers devised an elegant electrochemical biosensor able to rapidly and simultaneously measure the concentration of both forms of HSA - glycated and non-glycated. The detection limits she demonstrates exceeds the requirements for intermediate-term glycemic control monitoring in diabetes patients at 5 and 1 nM for albumin and its glycated forms, respectively.

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© 2020, Reginald M. Penner