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Hitachi F4500 fluorescence spectrometer

Scan speeds of up to 30,000 nm/min and wavelength drive rates of 60,000 nm/min provide rapid scans for both excitation and emission monochromators. Wavelength range: 200 ~ 900 nm, resolution 1.0 nm. Minimum sample volume 0.6 mL (with use of standard 10 mm cell).

Useful reading:

Quantum yield determination pdf

Excitation and emission spetroscopy fundamentals (by B. Herman, J.R. Lakowicz, D.B. Murphy, T.J.Fellers and M.W. Davidson for Olympus) pdf

Fluorescence spectroscopy (by Perkin Elmer) pdf

 

lab instructions

user guide