In the past 16 years, single molecule localization microscopy has revolutionized bioimaging with the super resolution. Through a completely different approach, a more recent technology Expansion Microscopy elevated the resolution again using expandable hydrogel. This talk will cover how Shi lab used novel chemical labels to combine these two microscopy methods to develop Label-Retention Expansion Microscopy (LR-ExM). This method achieved ultra-resolution and high labeling efficiency, which allows scientists to visualize individual proteins, genes, and RNA molecules in biological complexes.