Abstract: Modern biocatalysis relies on enzyme immobilization to operate at scale. Enzyme immobilization requires extensive optimization that is both time consuming and expensive. To solve this problem, a suite of 3D-printed tools was developed to miniaturize and parallelize immobilization experiments. In addition to 3D printing for biocatalysis, this work explores the use of microcontrollers to reduce the burden of repetitive laboratory tasks through automation. An electronic circuit was developed to actively protect oil-sealed vacuum pumps without human intervention. This work exemplifies the ability of 3D printing and electronics to translate a researcher’s imagination into functional and high performing instruments without waiting for the commercial market to respond.