Abstract: Solid-to-liquid state dynamic nuclear polarization (DNP) affords a sensitivity gain sufficient to enable single scan acquisition of NMR spectra at low concentration, of insensitive nuclei or of nuclei with low natural abundance. Combined with a rapid injection and mixing system, it becomes possible to obtain time-resolved data characterizing dynamic processes far from equilibrium. Kinetics and intermediates can be observed in various types of reactions, including enzyme catalyzed reactions of small-molecule substrates, fast organic reactions and polymerization reactions.